Identification, Cloning, and Characterization of l-Phenylserine Dehydrogenase from Pseudomonas syringae NK-15

Table 2

Effect of various compounds on the activity of L-phenylserine dehydrogenase.

Compound	Concentration	Relative activity
	(mM)	(%)
None	—	100
p-Chloromercuribenzoate	0.05	0
N-Ethylmaleimide	1	89
Iodoacetamide	1	91
EDTA^a	1	94
HgCl2	0.01	21
CuSO4	1	110
ZnSO4	1	92
MnSO4	1	110
MgSO4	1	100
BaCl2	1	99
NiCl2	1	95
CoCl2	1	94
CaCl2	1	93
KCl	187	87

L-Phenylserine dehydrogenase activity was determined in reaction mixtures containing the indicated additive, 20 mM DL-threo-𝛽-phenylserine and 2.5 mM NAD⁺ in 0.2 M glycine-KCl-KOH buffer (pH 10.5) at 30^∘C.
^aEthylenediaminetetraacetic acid.