Identification, Cloning, and Characterization of l-Phenylserine Dehydrogenase from Pseudomonas syringae NK-15

Table 3

Substrate specificity of L-phenylserine dehydrogenase.

Compound	Concentration	Relative activity
	(mM)	(%)
DL-threo-𝛽-phenylserine	20	100
D-threo-𝛽-phenylserine	10	0
DL-erythro-𝛽-phenylserine	20	840
DL-threo-(2-thienyl)serine	20	530
(S)-phenylethanol	10	6.9
DL-hydroxynorvaline	20	0
L-threonine	10	0
D-threonine	10	0
L-allo-threonine	10	0
D-allo-threonine	10	0
L-serine	10	0
D-serine	10	0
L-phenylalanine	10	0
D-phenylalanine	10	0

L-Phenylserine dehydrogenase activity was determined in reaction mixtures containing 10 or 20 mM substrate, as indicated, and 1.0 mM NAD⁺ in 0.2 M glycine-KCl-KOH buffer (pH 10.5) at 30^∘C.