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Enzyme Research
Volume 2010, Article ID 854526, 8 pages
Research Article

High-Yield Endoglucanase Production by Trichoderma harzianum IOC-3844 Cultivated in Pretreated Sugarcane Mill Byproduct

1Renewable Energy Division, Research and Development Center, Petrobras, Avenue Horácio Macedo, 950 Ilha do Fundão, 21941-915 Rio de Janeiro, RJ, Brazil
2School of Chemistry, Federal University of Rio de Janeiro, 21945-970 Rio de Janeiro, RJ, Brazil

Received 26 May 2010; Revised 15 July 2010; Accepted 12 August 2010

Academic Editor: J. M. S. Cabral

Copyright © 2010 Aline Machado de Castro et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The low-cost production of cellulolytic complexes presenting high action at mild conditions and well-balanced cellulase activities is one of the major bottlenecks for the economical viability of the production of cellulosic ethanol. In the present paper, the filamentous fungus Trichoderma harzianum IOC-3844 was used for the production of cellulases from a pretreated sugarcane bagasse (namely, cellulignin), by submerged fermentation. This fungal strain produced high contents of endoglucanase activity (6,358 U·) after 72 hours of process, and further relevant -glucosidase and FPase activities (742 and 445 U·, resp.). The crude enzyme extract demonstrated appropriate characteristics for its application in cellulose hydrolysis, such as high thermal stability at up to , accessory xylanase activity, and absence of proteolytic activity towards azocasein. This strain showed, therefore, potential for the production of complete cellulolytic complexes aiming at the saccharification of lignocellulosic materials.