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Enzyme Research
Volume 2010 (2010), Article ID 951472, 5 pages
http://dx.doi.org/10.4061/2010/951472
Research Article

The –SH Protection Method for Determining Accurate Kd Values for Enzyme-Coenzyme Complexes of NAD+-Dependent Glutamate Dehydrogenase and Engineered Mutants: Evidence for Nonproductive NADPH Complexes

School of Biomolecular and Biomedical Science, Conway Institute, University College Dublin, Belfield, Dublin 4, Ireland

Received 13 January 2010; Accepted 8 May 2010

Academic Editor: David Ballou

Copyright © 2010 Joanna Griffin and Paul C. Engel. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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