Research Article
The –SH Protection Method for Determining Accurate Kd Values for Enzyme-Coenzyme Complexes of NAD+-Dependent Glutamate Dehydrogenase and Engineered Mutants: Evidence for Nonproductive NADPH Complexes
Table 1
Comparison of limiting values of the first-order rate constants () for saturating levels of DTNB for wild-type GDH and mutant variants, and of dissociation constants governing binding of DTNB to the enzymes ().
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