Optimization of a Cytochrome-P450-Monooxygenase-1A-Mediated EROD Assay in the Cape Hake Species Merluccius capensis and Merluccius paradoxus (Pisces)
Figure 4
Assay optimization curves for M. capensis/M. paradoxus showing influence of pH (a) and temperature (b) on EROD activity in the pellet 2 fraction. Activities were determined spectrophotometrically at 572 nm and expressed as a % of maximal activity. Data points represent mean values ± SD (). Standard deviation bars are indicated but cannot be observed in cases were standard deviations were ≤0.0047.
