Expression, Purification, and Characterisation of Dehydroquinate Synthase from Pyrococcus furiosus
Figure 1
SDS-PAGE analysis of PfuDHQS. (a) Protein obtained from cell lysis under low-salt conditions. (1) Marker; (2) total crude; (3) soluble crude (supernatant obtained from centrifugation of the total crude); (4) insoluble crude (resuspended pellet, in 6 M urea, obtained from centrifugation of the total crude); (5) soluble heat treated (supernatant obtained from heat-treated soluble crude). (b) Protein obtained from cell lysis under high-salt conditions. (1) Marker; (2) total crude; (3) soluble heat treated; (4) insoluble heat treated; (5) anion exchange.
