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Enzyme Research
Volume 2011, Article ID 157294, 7 pages
http://dx.doi.org/10.4061/2011/157294
Research Article

Biochemical and Structural Characterization of Amy1: An Alpha-Amylase from Cryptococcus flavus Expressed in Saccharomyces cerevisiae

1Laboratório de Biotecnologia, Universidade Federal de São João del-Rei, 35501-296 Divinópolis, MG, Brazil
2Departamento de Bioquímica e Imunologia, Escola de Medicina, Universidade de São Paulo, 14049-900 Ribeirão Preto, SP, Brazil
3Laboratório de Biofísica, Instituto de Biologia, Universidade de Brasília, 70910-900 Brasília, DF, Brazil
4Laboratório de Biologia Molecular, Instituto de Biologia, Universidade de Brasília, 70910-900 Brasília, DF, Brazil
5Laboratório de Enzimologia, Departamento de Ciências Fisiológicas (ICB), Universidade Federal de Goiás, 74001-970 Goiânia, GO, Brazil

Received 31 October 2010; Accepted 18 December 2010

Academic Editor: Alane Beatriz Vermelho

Copyright © 2011 Alexsandro Sobreira Galdino et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. M. J. E. C. Van Der Maarel, B. Van Der Veen, J. C. M. Uitdehaag, H. Leemhuis, and L. Dijkhuizen, “Properties and applications of starch-converting enzymes of the α-amylase family,” Journal of Biotechnology, vol. 94, no. 2, pp. 137–155, 2002. View at Publisher · View at Google Scholar · View at Scopus
  2. B. Henrissat, “A classification of glycosyl hydrolases based on amino acid sequence similarities,” Biochemical Journal, vol. 280, no. 2, pp. 309–316, 1991. View at Google Scholar · View at Scopus
  3. B. Henrissat and A. Bairoch, “Updating the sequence-based classification of glycosyl hydrolases,” Biochemical Journal, vol. 316, no. 2, pp. 695–696, 1996. View at Google Scholar · View at Scopus
  4. R. Gupta, P. Gigras, H. Mohapatra, V. K. Goswami, and B. Chauhan, “Microbial α-amylases: a biotechnological perspective,” Process Biochemistry, vol. 38, no. 11, pp. 1599–1616, 2003. View at Publisher · View at Google Scholar · View at Scopus
  5. J. J. Wilson and W. M. Ingledew, “Isolation and characterization of Schwanniomyces alluvius amylolytic enzymes,” Applied and Environmental Microbiology, vol. 44, no. 2, pp. 301–307, 1982. View at Google Scholar · View at Scopus
  6. R. De Mot and H. Verachtert, “Secretion of α-amylase and multiple forms of glucoamylase by the yeast Trichosporon pullulans,” Canadian Journal of Microbiology, vol. 32, no. 1, pp. 47–51, 1986. View at Google Scholar · View at Scopus
  7. K. J. Wanderley, F. A. G. Torres, L. M. P. Moraes, and C. J. Ulhoa, “Biochemical characterization of α-amylase from the yeast Cryptococcus flavus,” FEMS Microbiology Letters, vol. 231, no. 2, pp. 165–169, 2004. View at Publisher · View at Google Scholar · View at Scopus
  8. A. S. Galdino, C. J. Ulhoa, L. M. P. Moraes, M. V. Prates, C. Bloch, and F. A. G. Torres, “Cloning, molecular characterization and heterologous expression of AMY1, an α-amylase gene from Cryptococcus flavus,” FEMS Microbiology Letters, vol. 280, no. 2, pp. 189–194, 2008. View at Publisher · View at Google Scholar · View at Scopus
  9. M. M. Bradford, “A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding,” Analytical Biochemistry, vol. 72, no. 1-2, pp. 248–254, 1976. View at Google Scholar · View at Scopus
  10. U. K. Laemmli, “Cleavage of structural proteins during the assembly of the head of bacteriophage T4,” Nature, vol. 227, no. 5259, pp. 680–685, 1970. View at Publisher · View at Google Scholar · View at Scopus
  11. H. Blum, H. Bier, and H. Gross, “Improved silver staining of plants proteins, RNA and DNA in polyacrylamide gels,” Electrophoresis, vol. 8, pp. 93–99, 1987. View at Google Scholar
  12. H. Fuwa, “A new method for microdetermination of amylase activity by the use of amylose as the substrate,” Journal of Biochemistry, vol. 41, no. 5, pp. 583–603, 1954. View at Google Scholar
  13. G. L. Miller, “Use of dinitrosalicylic acid reagent for determination of reducing sugar,” Analytical Chemistry, vol. 31, no. 3, pp. 426–428, 1959. View at Google Scholar · View at Scopus
  14. M. Lato, B. Brunelli, G. Ciuffini, and T. Mezzetti, “Thin-layer chromatography of carbohydrates on silica gel impregnated with sodium acetate, monosodium phosphate and disodium phosphate,” Journal of Chromatography A, vol. 39, pp. 407–417, 1969. View at Google Scholar · View at Scopus
  15. G. Bohm, R. Muhr, and R. Jaenicke, “Quantitative analysis of protein far UV circular dichroism spectra by neural networks,” Protein Engineering, vol. 5, no. 3, pp. 191–195, 1992. View at Google Scholar · View at Scopus
  16. H. Iefuji, M. Chino, M. Kato, and Y. Iimura, “Raw-starch-digesting and thermostable α-amylase from the yeast Cryptococcus sp. S-2: purification, characterization, cloning and sequencing,” Biochemical Journal, vol. 318, no. 3, pp. 989–996, 1996. View at Google Scholar · View at Scopus
  17. C. E. Goto, E. P. Barbosa, L. C. L. Kistner, F. G. Moreira, V. Lenartovicz, and R. M. Peralta, “Production of amylase by Aspergillus fumigatus utilizing α-methyl-D-glycoside, a synthetic analogue of maltose, as substrate,” FEMS Microbiology Letters, vol. 167, no. 2, pp. 139–143, 1998. View at Publisher · View at Google Scholar · View at Scopus
  18. H. K. Kang, J. H. Lee, D. Kim et al., “Cloning and expression of Lipomyces starkeyiα-amylase in Escherichia coli and determination of some of its properties,” FEMS Microbiology Letters, vol. 233, no. 1, pp. 53–64, 2004. View at Publisher · View at Google Scholar · View at Scopus
  19. A. J. C. Steyn, J. Marmur, and I. S. Pretorius, “Cloning, mapping and characterization of a genomic copy of the Lipomyces kononenkoaeα-amylase-encoding gene (LKA1),” Yeast, vol. 12, no. 10, pp. 925–937, 1996. View at Publisher · View at Google Scholar · View at Scopus
  20. H. Li, Z. Chi, X. Wang, X. Duan, L. Ma, and L. Gao, “Purification and characterization of extracellular amylase from the marine yeast Aureobasidium pullulans N13d and its raw potato starch digestion,” Enzyme and Microbial Technology, vol. 40, no. 5, pp. 1006–1012, 2007. View at Publisher · View at Google Scholar · View at Scopus
  21. J. A. Prieto, B. R. Bort, J. Martínez, F. Randez-Gil, C. Buesa, and P. Sanz, “Purification and characterization of a new alpha-amylase of intermediate thermal stability from the yeast Lipomyces kononenkoae,” Biochemistry and Cell Biology, vol. 73, no. 1-2, pp. 41–49, 1995. View at Google Scholar · View at Scopus
  22. A. Takeuchi, A. Shimizu-Ibuka, Y. Nishiyama et al., “Purification and characterization of an α-amylase of Pichia burtonii isolated from the traditional starter "murcha" in Nepal,” Bioscience, Biotechnology and Biochemistry, vol. 70, no. 12, pp. 3019–3024, 2006. View at Publisher · View at Google Scholar · View at Scopus
  23. S. Murakami, H. Nishimoto, Y. Toyama et al., “Purification and characterization of two alkaline, thermotolerant α-amylases from Bacillus halodurans 38C-2-1 and expression of the cloned gene in Escherichia coli,” Bioscience, Biotechnology and Biochemistry, vol. 71, no. 10, pp. 2393–2401, 2007. View at Publisher · View at Google Scholar · View at Scopus
  24. F. Moranelli, M. Yaguchi, G. B. Calleja, and A. Nasim, “Purification and characterization of the extracellular alpha-amylase activity of the yeast Schwanniomyces alluvius,” Biochemistry and Cell Biology, vol. 65, no. 10, pp. 899–908, 1987. View at Google Scholar · View at Scopus
  25. M. Ballschmiter, O. Fütterer, and W. Liebl, “Identification and characterization of a novel intracellular alkaline α-amylase from the hyperthermophilic bacterium Thermotoga maritima MSB8,” Applied and Environmental Microbiology, vol. 72, no. 3, pp. 2206–2211, 2006. View at Publisher · View at Google Scholar · View at Scopus
  26. J. L. Glymph and F. J. Stutzenberger, “Production, purification, and characterization of α-amylase from Thermomonospora curvata,” Applied and Environmental Microbiology, vol. 34, no. 4, pp. 391–397, 1977. View at Google Scholar · View at Scopus
  27. J. L. Marco, L. A. Bataus, F. F. Valencia, C. J. Ulhoa, S. Astolfi-Filho, and C. R. Felix, “Purification and characterization of a truncated Bacillus subtilisα-amylase produced by Escherichia coli,” Applied Microbiology and Biotechnology, vol. 44, no. 6, pp. 746–752, 1996. View at Publisher · View at Google Scholar · View at Scopus