Research Article
Biochemical and Structural Characterization of Amy1: An Alpha-Amylase from Cryptococcus flavus Expressed in Saccharomyces cerevisiae
Table 1
Summary of the purification steps of Amy1.
| | Step | Total activity (U) | Total protein (mg × 10-3) | Specific activity (U/mg × 10-3) | Purification (fold) | Yield (%) |
| | Supernatant | 1922.2 | 1.750 | 1098.5 | 1.00 | 100.0 | | Q-Sepharose | 490.8 | 0.120 | 4090.0 | 3.72 | 25.4 | | Sephacryl S-200HR | 199.9 | 0.048 | 4164.5 | 3.79 | 10.3 |
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