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Enzyme Research
Volume 2011, Article ID 167248, 7 pages
http://dx.doi.org/10.4061/2011/167248
Research Article

Production and Partial Characterization of Cellulases from Trichoderma sp. IS-05 Isolated from Sandy Coastal Plains of Northeast Brazil

Centro de Ciências Agrárias, Ambientais e Biológicas (CCAAB), Universidade Federal do Recôncavo da Bahia (UFRB), 44380-000 Cruz das Almas, BA, Brazil

Received 13 January 2011; Revised 30 May 2011; Accepted 29 June 2011

Academic Editor: Francisco Gírio

Copyright © 2011 Jackeline Pereira Andrade et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

This study evaluated the production of cellulolytic enzymes by Trichoderma sp. IS-05 strain, isolated from sand dunes, according to its ability to grow on cellulose as carbon source. Wheat bran was tested as the carbon source and peptone tested as the nitrogen source. Different concentrations of carbon and nitrogen were tested using a factorial design to identify optimal cellulase activity production. The results showed that media containing wheat bran 4.0% (w/v) and peptone 0.25% (w/v) lead to the highest production, 564.0 U L1 of cellulase, obtained after 2 days of fermentation. The pH and temperature profile showed optimal activity at pH 3.0 and 60C. As for thermostability, the cellulase was most tolerant at 60C, retaining more than 59.6% of maximal activity even after 4 hours of incubation. The combination of acid pH, high temperature tolerance, and production of cellulase from agro-industrial residues by Trichoderma sp. IS-05 offers possibilities condition for the biomass hydrolysis process to produce bioethanol.