Identification of a Functional Type IA Topoisomerase, LdTopIIIβ, from Kinetoplastid Parasite Leishmania donovani
Figure 4
Functional complementation of LdTopIIIβ. (a) S. cerevisiae top3Δ strain was transformed with a vector pVT100U and the vector carrying wild-type LdTopIIIβ. Transformed cells were streaked on solid synthetic minimal media and incubated at 28°C. Ten-fold serial dilutions of exponentially growing wild-type strain, top3Δ strain, top3Δ strain harboring an empty vector, or top3Δ strain harboring plasmid encoded LdTopIIIβ, as indicated on the right, grown on the plate. (b) Growth rate of the above-described strains were measured in the liquid synthetic medium and OD600 was plotted against time. Results represent the means ± standard errors of three independent experiments.
