Identification of a Functional Type IA Topoisomerase, LdTopIIIβ, from Kinetoplastid Parasite Leishmania donovani
Figure 5
Complementation assay with mutant LdTopIIIβ. (a) Topoisomerase III mutant yeast strain was transformed with the plasmid containing wild-type (c), active site mutant (d) and C-terminal deletion construct (e) of LdTopIIIβ, separately. Transformed cells were streaked on solid synthetic minimal media and incubated at 30°C. (b) Complementation assay as described above carried out in liquid synthetic medium and OD600 plotted against time. Results represent the means ± standard errors of three independent experiments.
