Research Article

Identification of a Functional Type IA Topoisomerase, LdTopIIIβ, from Kinetoplastid Parasite Leishmania donovani

Figure 6

DNA relaxation assay by recombinant LdTopIIIβ. (a) Negatively super coiled DNA was incubated with 1, 2, 3, 4, 5, 7 and 10 μL of recombinant LdTopIIIβ containing lysate for 30 min (lanes 2–8). Lane 1 is the DNA control. (b) DNA relaxation assay carried out with recombinant LdTopIIIβ (lane 2), and empty vector containing lysate (lane 3). Lane 1 is the DNA control.
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230542.fig.006b
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