Characterization of the Recombinant Thermostable Lipase (Pf2001) from Pyrococcus furiosus: Effects of Thioredoxin Fusion Tag and Triton X-100
Figure 1
SDS-PAGE (a) and zymography (b), on a 15% polyacrylamide gel, of expressed and purified protein encoded by the PF2001Δ60 gene. (1) soluble extract; (2) flow-through; (3) recombinant lipase eluted with 150 mM imidazole; (4) lipase treated with enterokinase to remove the thioredoxin tag.
