Characterization of the Recombinant Thermostable Lipase (Pf2001) from Pyrococcus furiosus: Effects of Thioredoxin Fusion Tag and Triton X-100
Figure 3
Thermal stability of the purified enzyme, with (a) and without (b) the thioredoxin tag. The enzyme samples in phosphate buffer (50 mM pH 7.0) containing 0.1% gum arabic and 0.4% Triton X-100 were incubated at the indicated temperatures for 5 min, 30 min, 2, and 6 h. The residual enzyme activity was assayed at 70°C, pH 7.0, using the substrate MUF-Hep.
