Characterization of the Recombinant Thermostable Lipase (Pf2001) from <i>Pyrococcus furiosus</i>: Effects of Thioredoxin Fusion Tag and Triton X-100

<table class="table-group" id="tab1"><tr><td><table class="table"><tr><td class="thead-hr" colspan="6"><hr/></td></tr><tr class="thead"><td align="left"></td><td align="center">Total protein (mg)</td><td align="center">Total activity (U)</td><td align="center">Specific activity (U/mg)</td><td align="center">Yield (%)</td><td align="center">Purification fold</td></tr><tr><td class="thead-hr" colspan="6"><hr/></td></tr><tr><td align="left">Soluble Ext.</td><td align="center">16.9</td><td align="center">22.5</td><td align="center">1.34</td><td align="center">100</td><td align="center">—</td></tr><tr><td align="left">Ni-NTA</td><td align="center">2.28</td><td align="center">20.4</td><td align="center">8.9</td><td align="center">89.8</td><td align="center">6.63</td></tr><tr class="table-tr"><td colspan="6"><hr class="tbody-hr"/></td></tr></table></td></tr></table>

Summary of the purification of the recombinant TRX-PF2001Δ60 from <i>P. furiosus</i>.

Enzyme Research

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Table 1

Table 1: Characterization of the Recombinant Thermostable Lipase (Pf2001) from <i>Pyrococcus furiosus</i>: Effects of Thioredoxin Fusion Tag and Triton X-100 

Table 1 | Characterization of the Recombinant Thermostable Lipase (Pf2001) from Pyrococcus furiosus: Effects of Thioredoxin Fusion Tag and Triton X-100 