Research Article
Characterization of the Recombinant Thermostable Lipase (Pf2001) from Pyrococcus furiosus: Effects of Thioredoxin Fusion Tag and Triton X-100
Table 2
Effects of metal ions, chemicals, and detergents on PF2001Δ60 lipase activity. The enzyme (0.5 μg) was incubated at 70°C in 50 mM sodium phosphate buffer (pH 7.0) in the presence of the tested agent using MUF-Hep as substrate. After 10 min, the mixture was assayed for residual activity using the standard lipase assay. The activity of the control was considered 100%.
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