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Enzyme Research
Volume 2011, Article ID 368525, 12 pages
Research Article

Application of Asymetrical and Hoke Designs for Optimization of Laccase Production by the White-Rot Fungus Fomes fomentarius in Solid-State Fermentation

1Unité Enzymes et Bioconversion, Ecole Nationale d’Ingénieurs de Sfax, route de Soukra 3038 Sfax, Tunisia
2Laboratoire de Chimie Industrielle, Ecole Nationale d’Ingénieurs de Sfax, route de Soukra 3038 Sfax, Tunisia
3Laboratoire Microorganismes et Biomolécules Actives, Faculté des Sciences de Tunis, Campus Universitaire, 2092 Tunis, Tunisia

Received 3 February 2011; Revised 25 March 2011; Accepted 30 March 2011

Academic Editor: Alane Beatriz Vermelho

Copyright © 2011 Mohamed Neifar et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Statistical approaches were employed for the optimization of different cultural parameters for the production of laccase by the white rot fungus Fomes fomentarius MUCL 35117 in wheat bran-based solid medium. first, screening of production parameters was performed using an asymmetrical design 2533//16, and the variables with statistically significant effects on laccase production were identified. Second, inoculum size, CaCl2 concentration, CuSO4 concentration, and incubation time were selected for further optimization studies using a Hoke design. The application of the response surface methodology allows us to determine a set of optimal conditions (CaCl2, 5.5 mg/gs, CuSO4, 2.5 mg/gs, inoculum size, 3 fungal discs (6 mm Ø), and 13 days of static cultivation). Experiments carried out under these conditions led to a laccase production yield of 150 U/g dry substrate.