Improved Laccase Production by Trametes pubescens MB89 in Distillery Wastewaters

Table 1

Laccase synthesis with different carbon, nitrogen, lignin/cellulose sources, and phosphorus (𝑛=2).

		HLA* ± std dev (units/L)	Day of HLA	Increase (fold)
Carbon sources	Fructose	3160±179	9±0.6	1.7±0.09
	Glucose	3238±793	9±0.6	1.7±0.42
	Mannitol	1091±161	6±0.0	0.6±0.08
	Maltose	2755±188	9±0.0	1.5±0.10
	Sucrose	3239±781	9±0.6	1.7±0.41
	Cellobiose	3306±262	9±0.0	1.7±0.14
	Lactose	2933±164	10±0.6	1.5±0.09
Nitrogen sources	NH4NO3	1759±218	6±0.0	0.9±0.11
	NH4Cl	1521±290	5±0.0	0.8±0.15
	KNO3	1879±313	10±0.0	1.0±0.16
	H2NCNH2	1485±166	5±0.0	0.8±0.09
	Malt extract	2080±489	10±0.0	1.1±0.26
	Yeast extract	2243±35	5±0.0	1.2±0.02
	Peptone	3428±422	5±0.0	1.8±0.22
Lignin/cellulose	Cellulose	1289±220	8±0.6	0.7±0.12
	Bluegum	1919±206	10±0.0	1.0±0.11
	Rooibos	2031±452	9±0.6	1.1±0.24
	Bagasse	1788±288	9±0.0	0.9±0.15
Phosphorous source	H3PO4	1978±44	9±1.2	1.0±0.02
	Control	1899±38	10±0.6	1.0±0.02

*HLA: highest laccase activity.