Improved Laccase Production by Trametes pubescens MB89 in Distillery Wastewaters

Table 2

Laccase synthesis obtained with the addition of various reported inducers prior to inoculation or four days thereafter (𝑛=3).

Reported inducer	Added prior to inoculation			Added after four days
	HLA* (units/L)	Day of HLA	Increase (fold)	HLA (units/L)	Day of HLA	Increase (fold)
2,5-Xylidine	8419	2	3.7	2944	11	2.4
Ethanol	6701	20	2.9	292	6	0.2
Copper	5492	20	2.4	1044	13	0.9
4-Methylcatechol	1153	20	0.5	2253	13	1.9
n-hydroxyphthalimide	1636	14	0.7	2283	13	1.9
Gallic acid	3303	14	1.4	820	8	0.7
Tannic acid	3114	20	1.4	1111	13	0.9
Quercetin	2966	14	1.3	588	13	0.5
Syringic acid	1862	14	0.8	1404	11	1.2
Guaiacol	2580	16	1.1	1292	11	1.1
Dimethoxybenzyl alcohol	1939	16	0.8	1247	13	1.0
Phenol	2149	14	0.9	1270	11	1.0
Violuric acid	2035	14	0.9	1039	11	0.9
Phenol red	2601	12	1.1	663	13	0.5
Cellulose	2474	16	1.1	328	13	0.3
p-Coumaric acid	2370	14	1.0	1227	13	1.0
Rooibos	2119	16	0.9	372	13	0.3
o-Cresol	2064	12	0.9	558	11	0.5
Dichloroindophenol	127	12	0.1	795	8	0.7
Hydroxybenzotriazole	977	14	0.4	656	13	0.5
Cycloheximide	20	4	0.0	455	5	0.4
Control	2305	12	1.0	1214	13	1.0

*HLA: highest laccase activity.