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Enzyme Research
Volume 2011, Article ID 457392, 9 pages
Research Article

Multiresponse Optimization of Inoculum Conditions for the Production of Amylases and Proteases by Aspergillus awamori in Solid-State Fermentation of Babassu Cake

1Biotechnology Division, Research and Development Center, PETROBRAS. Avenide Horácio Macedo, 950. Ilha do Fundão, 21941-915 Rio de Janeiro, RJ, Brazil
2COPPE, Chemical Engineering Program, Federal University of Rio de Janeiro, 21941-972 Rio de Janeiro, RJ, Brazil
3Institute of Chemistry, Federal University of Rio de Janeiro, 21945-970 Rio de Janeiro, RJ, Brazil

Received 14 January 2011; Accepted 11 July 2011

Academic Editor: Elba P. S. Bon

Copyright © 2011 Aline Machado de Castro et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


This work aimed at investigating the simultaneous production of amylases and proteases by solid-state fermentation (SSF) of babassu cake using Aspergillus awamori IOC-3914. By means of experimental design techniques and the desirability function, optimum inoculum conditions (C/N ratio of propagation medium, inoculum age, and concentration of inoculum added to SSF medium) for the production of both groups of enzymes were found to be 25.8, 28.4 h, and 9.1 mg g−1, respectively. Significant influence of both initial C/N ratio and inoculum concentration was observed. Optimum amylolytic activities predicted by this multiresponse analysis were validated by independent experiments, thus indicating the efficacy of this approach.