Multiresponse Optimization of Inoculum Conditions for the Production of Amylases and Proteases by Aspergillus awamori in Solid-State Fermentation of Babassu Cake
Table 4
Optimum inoculation conditions and enzyme activities predicted by means of the global desirability function, applied to two scenarios: considering only the amylolytic activities and considering also the proteases activity.
Predicted parameters
SSF time
72 h
96 h
120 h
Simultaneous analysis of endoamylase, and exoamylase production
value
0.439
0.902
0.763
Optimum inoculum age (h)
21.1
28.4
20.2
Optimum C/N ratio
25.8
25.8
25.8
Optimum inoculum concentration (mg g−1)
7.8
9.1
1.9
Exoamylolytic activity in optimum condition (U g−1)
27.1
73.6
52.5
Endoamylolytic activity in optimum condition (U g−1)
67.8
115.6
99.2
Simultaneous analysis of endoamylase, exoamylase, and proteases production
value
0.549
0.524
0.613
Optimum inoculum age (h)
33.7
25.8
21.8
Optimum C/N ratio
25.8
14.6
21.0
Optimum inoculum concentration (mg g−1)
3.5
9.7
2.8
Exoamylolytic activity in optimum condition (U g−1)
42.3
40.3
53.0
Endoamylolytic activity in optimum condition (U g−1)
