Multiresponse Optimization of Inoculum Conditions for the Production of Amylases and Proteases by Aspergillus awamori in Solid-State Fermentation of Babassu Cake

Table 5

Enzyme activities obtained in fermentations carried out for 96 h to validate the inoculum conditions predicted by desirability analysis to be optimal (inoculum age of 28.4 h, C/N ratio of 25.8, and inoculum concentration of 9.1 mg g⁻¹).

Replicate	Activity (U g^−1)
	Exoamylase	Endoamylase	Protease
Smaller scale-1	45.0	135.4	7.6
Smaller scale-2	44.2	113.9	21.1
Smaller scale-3	53.4	90.3	19.8
Smaller scale-4	63.9	100.3	23.3
Smaller scale-5	59.5	71.7	13.1
Larger scale	60.7	119.3	17.3
Overall mean	55.4	104.3	17.0
Overall SD	7.6	24.1	5.5
Values predicted by means of the desirability function	73.6	115.6	—