Fluorescence Polarization Binding Assay for Aspergillus fumigatus Virulence Factor UDP-Galactopyranose Mutase

Figure 3

FP assay design. (a) Binding of the FP probe to AfUGM leads to polarized fluorescence. (b) Displacement of the FP probe from AfUGM by inhibitor results in depolarized fluorescence.

(a)

(b)