Fluorescence Polarization Binding Assay for <i>Aspergillus fumigatus</i> Virulence Factor UDP-Galactopyranose Mutase

<table class="table-group" id="tab2"><tr><td><table class="table"><tr><td class="thead-hr" colspan="3"><hr/></td></tr><tr class="thead"><td align="left">Ligand</td><td align="center"><svg height="14.375" id="M49" style="vertical-align:-3.24037pt" version="1.1" viewbox="0 0 19.137501 14.375" width="19.137501" xmlns="http://www.w3.org/2000/svg">
<g transform="matrix(1.25,0,0,-1.25,0,14.375)">
<g transform="translate(72,-60.5)">
<text transform="matrix(1,0,0,-1,-71.95,63.79)">
<tspan style="font-size: 12.50px; " x="0" y="0">𝐾</tspan>
</text>
<text transform="matrix(1,0,0,-1,-62.42,60.66)">
<tspan style="font-size: 8.75px; " x="0" y="0">𝑑</tspan>
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</g>
</g>
</svg> for <i>Af</i>UGM (<i>μ</i>M)</td><td align="center"><svg height="14.375" id="M50" style="vertical-align:-3.24037pt" version="1.1" viewbox="0 0 19.137501 14.375" width="19.137501" xmlns="http://www.w3.org/2000/svg">
<g transform="matrix(1.25,0,0,-1.25,0,14.375)">
<g transform="translate(72,-60.5)">
<text transform="matrix(1,0,0,-1,-71.95,63.79)">
<tspan style="font-size: 12.50px; " x="0" y="0">𝐾</tspan>
</text>
<text transform="matrix(1,0,0,-1,-62.42,60.66)">
<tspan style="font-size: 8.75px; " x="0" y="0">𝑑</tspan>
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</g>
</g>
</svg> for <i>Mt</i>UGM (<i>μ</i>M)</td></tr><tr><td class="thead-hr" colspan="3"><hr/></td></tr><tr><td align="left">UDP</td><td align="center">9.0 ± 1.7</td><td align="center">15 ± 2</td></tr><tr><td align="left">UDP-Gal<i>p </i></td><td align="center">495 ± 66</td><td align="center">563 ± 75</td></tr><tr><td align="left"><b>7</b></td><td align="center">140 ± 9</td><td align="center">21 ± 1</td></tr><tr><td align="left"><b>8</b></td><td align="center">11 ± 0.4</td><td align="center">25 ± 2</td></tr><tr class="table-tr"><td colspan="3"><hr class="tbody-hr"/></td></tr></table></td></tr></table>

Enzyme Research

tab2

Table 2

Table 2: Fluorescence Polarization Binding Assay for <i>Aspergillus fumigatus</i> Virulence Factor UDP-Galactopyranose Mutase 

Enzyme Research

Fluorescence Polarization Binding Assay for Aspergillus fumigatus Virulence Factor UDP-Galactopyranose Mutase

Table 2