Glucose-6-Phosphate Dehydrogenase Regulation in Anoxia Tolerance of the Freshwater Crayfish Orconectes virilis

Table 2

Effect of incubation under conditions that stimulated the activities of protein phosphatases or protein kinases on the 𝐾_𝑚 values (mM) for NADP⁺ of crayfish hepatopancreas.

Condition:	Control 𝐾𝑚 (mM)	20 h Anoxic 𝐾𝑚 (mM)
Untreated	0.15±0.008	0.12±0.008^b
PP1	0.11±0.05	0.13±0.048
PP1/2A	0.22±0.035^a	0.19±0.033
Alkaline Phosphatase	0.38±0.002^a	0.38±0.036^a
PKA	0.017±0.005^a	0.014±0.001^a
PKG	0.023±0.002^a	0.020±0.003^a

Data are means ± SEM, 𝑛=3-4. aSignificantly different from the corresponding untreated value as determined using ANOVA and a post hoc Dunnett’s test, 𝑃<0.05; bsignificantly different from the corresponding control value, 𝑃<0.05.