Phosphorylcholine Phosphatase: A Peculiar Enzyme of Pseudomonas aeruginosa

Figure 5

(a) Amino acid sequence alignment of PchP with proteins from different organisms. The sequences analyzed were as follows: Paer, Pseudomonas aeruginosa PAO1; Psyr, Pseudomonas syringae pv. tomato str. DC3000; Pflu, Pseudomonas fluorescens Pf-5; Pent, Pseudomonas entomophila L48; Pput, Pseudomonas putida KT2440; Asp, Azospirillum sp. B510; Lnit, Lutiella nitroferrum 2002; Bamb, Burkholderia ambifaria MC40-6; Bcen, Burkholderia cenocepacia MC0-3; Bmul, Burkholderia multivorans ATCC 17616; Gzea, Gibberella zeae PH-1; Nhae, Nectria haematococca mpVI 77-13-4; Ggra, Glomerella graminicola M1.001; Lmac, Leptosphaeria maculans; Pter, Pyrenophora teres f. teres 0-1; Ptri, Pyrenophora tritici-repentis Pt-1C-BFP; Mory, Magnaporthe oryzae 70-15; Valb, Verticillium albo-atrum VaMs.102. The alignment was constructed using the program CLUSTAL-X [19]; (*) indicates identical residues, (:) indicates conserved residues, and (.) indicates semiconserved residues. The three catalytic motifs of the HAD superfamily are marked in red. (b) Evolutionary relationships of 18 taxa (linearized). The evolutionary history was inferred using the neighbor-joining method [20]. The optimal tree with the sum of branch length = 2.68745267 is shown. The phylogenetic tree was linearized assuming equal evolutionary rates in all lineages [21]. The clock calibration to convert distance to time was 0.002 (time/node height). The tree is drawn to scale, with branch lengths in the same units as those of the evolutionary distances used to infer the phylogenetic tree. The evolutionary distances were computed using the Poisson correction method [22] and are in units of amino acid substitutions per site. All positions containing gaps and missing data were eliminated from the dataset (complete deletion option). There were a total of 338 positions in the final dataset. Phylogenetic analyses were conducted in MEGA4 [23].