An Examination by Site-Directed Mutagenesis of Putative Key Residues in the Determination of Coenzyme Specificity in Clostridial <mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML"><mml:mrow><mml:msup><mml:mrow><mml:mtext>NAD</mml:mtext></mml:mrow><mml:mo mathvariant="bold">+</mml:mo></mml:msup></mml:mrow></mml:math>-Dependent Glutamate Dehydrogenase

<table>10% SDS PAGE illustrating overexpression and purification of D263K enzyme. Lane 1, molecular weight markers. Lane 2, overexpressed D263K. Lanes 3 and 4, purified D263K.</table>

Enzyme Research

fig3

Figure 3

Figure 3: An Examination by Site-Directed Mutagenesis of Putative Key Residues in the Determination of Coenzyme Specificity in Clostridial <mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML"><mml:mrow><mml:msup><mml:mrow><mml:mtext>NAD</mml:mtext></mml:mrow><mml:mo mathvariant="bold">+</mml:mo></mml:msup></mml:mrow></mml:math>-Dependent Glutamate Dehydrogenase 

Figure 3 | An Examination by Site-Directed Mutagenesis of Putative Key Residues in the Determination of Coenzyme Specificity in Clostridial NAD+-Dependent Glutamate Dehydrogenase 