An Examination by Site-Directed Mutagenesis of Putative Key Residues in the Determination of Coenzyme Specificity in Clostridial <mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML"><mml:mrow><mml:msup><mml:mrow><mml:mtext>NAD</mml:mtext></mml:mrow><mml:mo mathvariant="bold">+</mml:mo></mml:msup></mml:mrow></mml:math>-Dependent Glutamate Dehydrogenase

<table>Lineweaver-Burk plots of initial rates for wildtype Cs GDH • and mutants F238S ■, P262S ♦, and F238S/P262S ▲ as a function of varying NADH (a) and NADPH (b and c) concentrations at pH 8.0.</table>

Enzyme Research

fig4

Figure 4

Figure 4: An Examination by Site-Directed Mutagenesis of Putative Key Residues in the Determination of Coenzyme Specificity in Clostridial <mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML"><mml:mrow><mml:msup><mml:mrow><mml:mtext>NAD</mml:mtext></mml:mrow><mml:mo mathvariant="bold">+</mml:mo></mml:msup></mml:mrow></mml:math>-Dependent Glutamate Dehydrogenase 

Figure 4 | An Examination by Site-Directed Mutagenesis of Putative Key Residues in the Determination of Coenzyme Specificity in Clostridial NAD+-Dependent Glutamate Dehydrogenase 