Research ArticleAn Examination by Site-Directed Mutagenesis of Putative Key Residues in the Determination of Coenzyme Specificity in Clostridial
An Examination by Site-Directed Mutagenesis of Putative Key Residues in the Determination of Coenzyme Specificity in Clostridial NAD +
Table 3
Summary, for each enzyme at each pH, based on data from Table 2, of the extent of discrimination against NADPH and in favour of NADH. The figure, given in the third column, is calculated as the ratio of the catalytic efficiency, /, with NADH to that with NADPH. These ratios for each mutant are then in turn compared with the figure for the wildtype enzyme to obtain the figures in the fourth column, which indicate the extent to which the mutations have reversed the preference for NADH.
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