615803.fig.003
Figure 3: Mechanism of the hydrolysis reaction of ester bonds catalyzed by esterases and lipases. The catalytic triad and water are shown in black; the oxyanion hole residues are in blue; the substrate is in red. (a) Nucleophilic attack of the serine hydroxyl on the carbonyl carbon of the susceptible ester bond; (b) tetrahedral intermediate; (c) acyl-enzyme intermediate and nucleophilic attack by water; (d) tetrahedral intermediate; (e) free enzyme [90].