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Enzyme Research
Volume 2011, Article ID 615803, 16 pages
http://dx.doi.org/10.4061/2011/615803
Review Article

Production and Use of Lipases in Bioenergy: A Review from the Feedstocks to Biodiesel Production

1School of Chemistry, Federal University of Rio de Janeiro, 21941-970 Rio de Janeiro, RJ, Brazil
2Biotechnology Division, Research and Development Center, 21941-915 Petrobras, Brazil
3Institute of Chemistry, Federal University of Rio de Janeiro, 21941-970 Rio de Janeiro, RJ, Brazil

Received 30 January 2011; Accepted 28 April 2011

Academic Editor: Francisco Gírio

Copyright © 2011 Bernardo Dias Ribeiro et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. F. D. Gunstone and F. B. Padley, Lipid Technologies and Applications, Marcel Dekker, New York, NY, USA, 1997.
  2. J. J. Salas, J. Sánchez, U. S. Ramli, A. M. Manaf, M. Williams, and J. L. Harwood, “Biochemistry of lipid metabolism in olive and other oil fruits,” Progress in Lipid Research, vol. 39, no. 2, pp. 151–180, 2000. View at Publisher · View at Google Scholar · View at Scopus
  3. F. D. Gunstone, Vegetable Oils in Food Technology: Composition, Properties and Uses, Blackwell Publishing, Oxford, UK, 2002.
  4. M. F. Ali, B. M. E. Ali, and J. G. Speight, Handbook of Industrial Chemistry—Organic Chemicals, McGraw-Hill, New York, NY, USA, 2005.
  5. P. J. Eastmond and I. A. Graham, “Re-examining the role of the glyoxylate cycle in oilseeds,” Trends in Plant Science, vol. 6, no. 2, pp. 72–77, 2001. View at Publisher · View at Google Scholar · View at Scopus
  6. Food and Agriculture Organization of The United Nations (FAO), Food Outlook, FAO, New York, NY, USA, 2010.
  7. J. C. Melo, J. C. Teixeira, J. Z. Brito, J. G. A. Pacheco, and L. Stragevitch, “Produção de Biodiesel de Óleo de Oiticica,” in Proceedings of the I Congresso da Rede Brasileira de Tecnologia do Biodiesel, pp. 164–169, August 2006.
  8. M. J. Dabdoub, J. L. Bronze, and M. A. Rampin, “Biodiesel: visão crítica do status atual e perspectivas na academia e na indústria,” Química Nova, vol. 32, no. 3, pp. 776–792, 2009. View at Google Scholar · View at Scopus
  9. F. D. Gunstone, J. L. Harwood, and A. J. Dijkstra, The Lipid Handbook, CRC Press, Boca Raton, Fla, USA, 2007.
  10. L. M. P. Santos, “Nutritional and ecological aspects of buriti or aguaje (Mauritia flexuosa Linnaeus filius): a carotene-rich palm fruit from Latin America,” Ecology of Food and Nutrition, vol. 44, no. 5, pp. 345–358, 2005. View at Publisher · View at Google Scholar · View at Scopus
  11. F. Danieli, O óleo de abacate (Persea americana Mill) como matéria-prima para indústria alimentícia, Dissertation. thesis, University of São Paulo, Piracicaba, Brazil, 2006.
  12. Y. M. Choo, “Palm oil carotenoids,” Food and Nutrition Bulletin, vol. 15, no. 2, pp. 130–137, 1994. View at Google Scholar · View at Scopus
  13. M. C. T. Damaso, M. A. Passianoto, S. C. Freitas, D. M. G. Freire, R. C. A. Lago, and S. Couri, “Utilization of agroindustrial residues for lipase production by solid-state fermentation,” Brazilian Journal of Microbiology, vol. 39, no. 4, pp. 676–681, 2008. View at Publisher · View at Google Scholar · View at Scopus
  14. E. Wolski, E. Menusi, M. Mazutti et al., “Response surface methodology for optimization of lipase production by an immobilized newly isolated Penicillium sp,” Industrial & Engineering Chemistry Research, vol. 47, no. 23, pp. 9651–9657, 2008. View at Publisher · View at Google Scholar
  15. V. K. Garlapati, P. R. Vundavilli, and R. Banerjee, “Evaluation of lipase production by genetic algorithm and particle swarm optimization and their comparative study,” Applied Biochemistry and Biotechnology, vol. 162, no. 5, pp. 1350–1361, 2010. View at Publisher · View at Google Scholar
  16. M. Singh, K. Saurav, N. Srivastava, and K Kannabiran, “Lipase production by Bacillus subtilis OCR-4 in solid state fermentation using ground nut oil cakes as substrates,” Current Research Journal of Biological Sciences, vol. 2, no. 4, pp. 241–245, 2010. View at Google Scholar
  17. T. F. C. Salum, P. Villeneuve, B. Barea et al., “Synthesis of biodiesel in column fixed-bed bioreactor using the fermented solid produced by Burkholderia cepacia LTEB11,” Process Biochemistry, vol. 45, no. 8, pp. 1348–1354, 2010. View at Publisher · View at Google Scholar · View at Scopus
  18. S. Y. Sun and Y. Xu, “Solid-state fermentation for “whole-cell synthetic lipase” production from Rhizopus chinensis and identification of the functional enzyme,” Process Biochemistry, vol. 43, no. 2, pp. 219–224, 2008. View at Publisher · View at Google Scholar · View at Scopus
  19. R. Bussamara, A. M. Fuentefria, E. S. D. Oliveira et al., “Isolation of a lipase-secreting yeast for enzyme production in a pilot-plant scale batch fermentation,” Bioresource Technology, vol. 101, no. 1, pp. 268–275, 2010. View at Publisher · View at Google Scholar · View at Scopus
  20. S. Kumar, N. Katiyar, P. Ingle, and S. Negi, “Use of evolutionary operation (EVOP) factorial design techniques to develop a bioprocess using grease waste as substrate for lipase production,” Bioresource Technology, vol. 102, no. 7, pp. 4909–4912, 2011. View at Google Scholar
  21. Q. Li, W. Du, and D. Liu, “Perspectives of microbial oils for biodiesel production,” Applied Microbiology and Biotechnology, vol. 80, no. 5, pp. 749–756, 2008. View at Publisher · View at Google Scholar · View at Scopus
  22. M. G. Morais and J. A. V. Costa, “Fatty acids profile of microalgae cultived with carbon dioxide,” Ciência e Agrotecnologia, vol. 32, no. 4, pp. 1245–1251, 2008. View at Google Scholar · View at Scopus
  23. G. Knothe, J. V. Gerpen, and J. Krahl, The Biodiesel Handbook, AOCS Press, Champaign, Ill, USA, 2005.
  24. Y. B. Zuo, A. W. Zeng, X. G. Yuan, and K. T. Yu, “Extraction of soybean isoflavones from soybean meal with aqueous methanol modified supercritical carbon dioxide,” Journal of Food Engineering, vol. 89, no. 4, pp. 384–389, 2008. View at Publisher · View at Google Scholar · View at Scopus
  25. L. N. Ceci, D. T. Constenla, and G. H. Crapiste, “Oil recovery and lecithin production using water degumming sludge of crude soybean oils,” Journal of the Science of Food and Agriculture, vol. 88, no. 14, pp. 2460–2466, 2008. View at Publisher · View at Google Scholar · View at Scopus
  26. U. T. Bornscheuer, “Microbial carboxyl esterases: classification, properties and application in biocatalysis,” FEMS Microbiology Reviews, vol. 26, no. 1, pp. 73–81, 2002. View at Publisher · View at Google Scholar · View at Scopus
  27. K. E. Jaeger, B. W. Dijkstra, and M. T. Reetz, “Bacterial biocatalysts: molecular biology, three-dimensional structures, and biotechnological applications of lipases,” Annual Review of Microbiology, vol. 53, pp. 315–351, 1999. View at Publisher · View at Google Scholar · View at Scopus
  28. R. Sharma, Y. Chisti, and U. C. Banerjee, “Production, purification, characterization, and applications of lipases,” Biotechnology Advances, vol. 19, no. 8, pp. 627–662, 2001. View at Publisher · View at Google Scholar · View at Scopus
  29. H. F. Castro, A. A. Mendes, and J. C. Santos, “Modificação de óleos e gorduras por biotransformação,” Química Nova, vol. 27, no. 1, pp. 146–156, 2004. View at Google Scholar · View at Scopus
  30. D. M. G. Freire and L. R. Castilho, “Lipases em Biocatálise,” in Enzimas em Biotecnologia. Produção, Aplicações e Mercado, vol. 1, pp. 369–385, Interciência, Rio de Janeiro, Brazil, 2008. View at Google Scholar
  31. K. E. Jaeger and T. Eggert, “Lipases for biotechnology,” Current Opinion in Biotechnology, vol. 13, no. 4, pp. 390–397, 2002. View at Publisher · View at Google Scholar · View at Scopus
  32. P. Villeneuve, “Plant lipases and their applications in oils and fats modification,” European Journal of Lipid Science and Technology, vol. 105, no. 6, pp. 308–317, 2003. View at Publisher · View at Google Scholar · View at Scopus
  33. E. D. C. Cavalcanti, F. M. Maciel, P. Villeneuve, R. C. A. Lago, O. L. T. Machado, and D. M. G. Freire, “Acetone powder from dormant seeds of Ricinus communis L: lipase activity and presence of toxic and allergenic compounds,” Applied Biochemistry and Biotechnology, vol. 137–140, no. 1–12, pp. 57–65, 2007. View at Publisher · View at Google Scholar · View at Scopus
  34. N. López, M. A. Pernas, L. M. Pastrana, A. Sánchez, F. Valero, and M. L. Rua, “Reactivity of pure Candida rugosa lipase isoenzymes (Lip1, Lip2, and Lip3) in aqueous and organic media. Influence of the isoenzymatic profile on the lipase performance in organic media,” Biotechnology Progress, vol. 20, no. 1, pp. 65–73, 2004. View at Publisher · View at Google Scholar · View at Scopus
  35. J. Pfeffer, S. Richter, J. Nieveler et al., “High yield expression of lipase A from Candida antarctica in the methylotrophic yeast Pichia pastoris and its purification and characterisation,” Applied Microbiology and Biotechnology, vol. 72, no. 5, pp. 931–938, 2006. View at Publisher · View at Google Scholar · View at Scopus
  36. M. L. M. Fernandes, N. Krieger, A. M. Baron, P. P. Zamora, L. P. Ramos, and D. A. Mitchell, “Hydrolysis and synthesis reactions catalysed by Thermomyces lanuginosa lipase in the AOT/Isooctane reversed micellar system,” Journal of Molecular Catalysis B, vol. 30, no. 1, pp. 43–49, 2004. View at Publisher · View at Google Scholar · View at Scopus
  37. Z.-Y. Shu, J.-K. Yang, and Y.-J. Yan, “Purification and characterization of a lipase from Aspergillus niger F044,” Chinese Journal of Biotechnology, vol. 23, no. 1, pp. 96–101, 2007. View at Publisher · View at Google Scholar · View at Scopus
  38. H. Ogino, S. Nakagawa, K. Shinya et al., “Purification and characterization of organic solvent-stable lipase from organic solvent-tolerant Pseudomonas aeruginosa LST-03,” Journal of Bioscience and Bioengineering, vol. 89, no. 5, pp. 451–457, 2000. View at Publisher · View at Google Scholar · View at Scopus
  39. E. Lesuisse, K. Schanck, and C. Colson, “Purification and preliminary characterization of the extracellular lipase of Bacillus subtilis 168, an extremely basic pH-tolerant enzyme,” European Journal of Biochemistry, vol. 216, no. 1, pp. 155–160, 1993. View at Google Scholar · View at Scopus
  40. J. F. M. Burkert, F. Maugeri, and M. I. Rodrigues, “Optimization of extracellular lipase production by Geotrichum sp. using factorial design,” Bioresource Technology, vol. 91, no. 1, pp. 77–84, 2004. View at Publisher · View at Google Scholar · View at Scopus
  41. R. R. Maldonado, Produção, purificação e caracterização da lipase de Geotrichum candidum obtida a partir de meios industriais, Disseration. thesis, University of Campinas, Campinas, Brazil, 2006.
  42. M. Abrimic, I. Lescic, T. Korica, L. Vitale, W. Saenger, and J. Pigac, “Purification and properties of extracellular lipase from Streptomyces rimosus,” Enzyme and Microbial Technology, vol. 25, no. 6, pp. 522–529, 1999. View at Publisher · View at Google Scholar · View at Scopus
  43. J. Destain, D. Roblain, and P. Thonart, “Improvement of lipase production from Yarrowia lipolytica,” Biotechnology Letters, vol. 19, no. 2, pp. 105–107, 1997. View at Publisher · View at Google Scholar · View at Scopus
  44. A. Aloulou, J. A. Rodriguez, D. Puccinelli et al., “Purification and biochemical characterization of the LIP2 lipase from Yarrowia lipolytica,” Biochimica et Biophysica Acta, vol. 1771, no. 2, pp. 228–237, 2007. View at Publisher · View at Google Scholar · View at Scopus
  45. A. I. S. Brigida, P. F. F. Amaral, L. R. B. Gonçalves, and M. A. Z. Coelho, “Characterization of an Extracellular lipase from Yarrowia lipolytica,” in Proceedings of the European Congress of Chemical Engineering 6, (ECCE '07), vol. 2, Norhaven Book, Copenhagen, Denmark, September 2007.
  46. H. Uhlig, Industrial Enzymes and Their Applications, John Wiley & Sons, New York, NY, USA, 1998.
  47. H. Abbas, A. Hiol, V. Deyris, and L. Comeau, “Isolation and characterization of an extracellular lipase from Mucor sp. strain isolated from palm fruit,” Enzyme and Microbial Technology, vol. 31, no. 7, pp. 968–975, 2002. View at Publisher · View at Google Scholar · View at Scopus
  48. T. Godfrey and S. West, Industrial Enzymology, The Macmillan Press, London, UK, 1996.
  49. P. J. Eastmond, “Cloning and characterization of the acid lipase from castor beans,” Journal of Biological Chemistry, vol. 279, no. 44, pp. 45540–45545, 2004. View at Publisher · View at Google Scholar · View at Scopus
  50. E. D. C. Cavalcanti, Avaliação da atividade lipásica da semente dormente de Ricinus communis, Disseration. thesis, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil, 2006.
  51. R. R. Costa Neto, Obtenção de ésteres alquilicos (Biodiesel) por via enzimática a partir de óleo de soja, Ph.D. thesis, Federal University of Santa Catarina, Florianópolis, Brazil, 2002.
  52. C. T. Hou and J. F. Shaw, Biocatalysis and Bioenergy, John Wiley & Sons, Hoboken, NJ, USA, 2008.
  53. A. Nag, Biofuels Refining and Performance, McGraw-Hill, New York, NY, USA, 2008.
  54. L. R. Castilho, C. M. S. Polato, E. A. Baruque, G. L. Sant'Anna Jr., and D. M. G. Freire, “Economic analysis of lipase production by Penicillium restrictum in solid-state and submerged fermentations,” Biochemical Engineering Journal, vol. 4, no. 3, pp. 239–247, 2000. View at Publisher · View at Google Scholar · View at Scopus
  55. L. A. I. Azeredo, P. M. Gomes, G. L. Sant'Anna Jr., L. R. Castilho, and D. M. G. Freire, “Production and regulation of lipase activity from Penicillium restrictum in submerged and solid-state fermentations,” Current Microbiology, vol. 54, no. 5, pp. 361–365, 2007. View at Publisher · View at Google Scholar · View at Scopus
  56. M. B. Palma, A. L. Pinto, A. K. Gombert et al., “Lipase production by Penicillium restrictum using solid waste of industrial babassu oil production as substrate,” Applied Biochemistry and Biotechnology, vol. 84–86, pp. 1137–1145, 2000. View at Google Scholar · View at Scopus
  57. M. L. E. Gutarra, E. D. C. Cavalcanti, D. M. G. Freire, L. R. Castilho, and G. L. Sant'Anna Jr., “Lipase production by solid-state fermentation: cultivation conditions and operation of tray and packed-bed bioreactors,” Applied Biochemistry and Biotechnology, vol. 121, no. 1–3, pp. 105–116, 2005. View at Google Scholar · View at Scopus
  58. M. L. E. Gutarra, M. G. Godoy, F. Maugeri, M. I. Rodrigues, D. M. G. Freire, and L. R. Castilho, “Production of an acidic and thermostable lipase of the mesophilic fungus Penicillium simplicissimum by solid-state fermentation,” Bioresource Technology, vol. 100, no. 21, pp. 5249–5254, 2009. View at Publisher · View at Google Scholar · View at Scopus
  59. E. D. C. Cavalcanti, M. L. E. Gutarra, D. M. G. Freire, L. R. Castilho, and G. L. Sant'Anna Jr., “Lipase production by solid-state fermentation in fixed-bed bioreactors,” Brazilian Archives of Biology and Technology, vol. 48, pp. 79–84, 2005. View at Google Scholar · View at Scopus
  60. M. Diluccio, F. Capra, N. P. Ribeiro, G. D. L. P. Vargas, D. M. G. Freire, and D. Oliveira, “Evaluation of lipase production by solid state fermentation by Penicillium simplicissimum using soy cake,” Applied Biochemistry and Biotechnology, vol. 113, pp. 173–180, 2004. View at Google Scholar
  61. M. G. Godoy, M. L. E. Gutarra, A. M. Castro, O. L. M. Tavares, and D. M. G. Freire, “Adding value to a toxic residue from the biodiesel industry: production of two distinct pool of lipases from Penicillium simplicissimum in castor bean waste,” Journal of Industrial Microbiology & Biotechnology. In press. View at Publisher · View at Google Scholar
  62. P. V. Rao, K. Jayaraman, and C. M. Lakshmanan, “Production of lipase by Candida rugosa in solid state fermentation. 1: determination of significant process variables,” Process Biochemistry, vol. 28, no. 6, pp. 385–389, 1993. View at Google Scholar · View at Scopus
  63. J. A. Rodriguez, J. C. Mateos, J. Nungaray et al., “Improving lipase production by nutrient source modification using Rhizopus homothallicus cultured in solid state fermentation,” Process Biochemistry, vol. 41, no. 11, pp. 2264–2269, 2006. View at Publisher · View at Google Scholar · View at Scopus
  64. J. C. M. Mateos Diaz, J. A. Rodríguez, S. Roussos et al., “Lipase from the thermotolerant fungus Rhizopus homothallicus is more thermostable when produced using solid state fermentation than liquid fermentation procedures,” Enzyme and Microbial Technology, vol. 39, no. 5, pp. 1042–1050, 2006. View at Publisher · View at Google Scholar · View at Scopus
  65. N. D. Mahadik, U. S. Puntambekar, K. B. Bastawde, J. M. Khire, and D. V. Gokhale, “Production of acidic lipase by Aspergillus niger in solid state fermentation,” Process Biochemistry, vol. 38, no. 5, pp. 715–721, 2002. View at Publisher · View at Google Scholar · View at Scopus
  66. N. R. Kamini, J. G. S. Mala, and R. Puvanakrishnan, “Lipase production from Aspergillus niger by solid-state fermentation using gingelly oil cake,” Process Biochemistry, vol. 33, no. 5, pp. 505–511, 1998. View at Publisher · View at Google Scholar · View at Scopus
  67. J. Cordova, M. Nemmaoui, M. Ismaïli-Alaoui et al., “Lipase production by solid state fermentation of olive cake and sugar cane bagasse,” Journal of Molecular Catalysis. B, vol. 5, no. 1–4, pp. 75–78, 1998. View at Publisher · View at Google Scholar · View at Scopus
  68. I. ul-Haq, S. Idrees, and M. I. Rajoka, “Production of lipases by Rhizopus oligosporous by solid-state fermentation,” Process Biochemistry, vol. 37, no. 6, pp. 637–641, 2002. View at Publisher · View at Google Scholar · View at Scopus
  69. D. A. Mitchell, B. K. Losane, A. Durand et al., “General principles of reactors design and operation for SSC,” in Solid Substrate Cultivation, H. Doelle, D. A. Mitchell, and C. E. Rols, Eds., pp. 115–139, Elsevier Applied Science, Amsterdam, The Netherlands, 1992. View at Google Scholar
  70. D. A. Mitchell, A. Pandey, P. Sangsurasak, and N. Krieger, “Scale-up strategies for packed-bed bioreactors for solid-state fermentation,” Process Biochemistry, vol. 35, no. 1-2, pp. 167–178, 1999. View at Publisher · View at Google Scholar · View at Scopus
  71. D. A. Mitchell, N. Krieger, D. M. Stuart, and A. Pandey, “New developments in solid-state fermentation II. Rational approaches to the design, operation and scale-up of bioreactors,” Process Biochemistry, vol. 35, no. 10, pp. 1211–1225, 2000. View at Publisher · View at Google Scholar · View at Scopus
  72. D. A. Mitchell, M. Berovic, and N. Krieger, “Overview of solid state bioprocessing,” Biotechnology Annual Review, vol. 8, pp. 183–225, 2002. View at Publisher · View at Google Scholar · View at Scopus
  73. F. D. H. Dalsenter, G. Viccini, M. C. Barga, D. A. Mitchell, and N. Krieger, “A mathematical model describing the effect of temperature variations on the kinetics of microbial growth in solid-state culture,” Process Biochemistry, vol. 40, no. 2, pp. 801–807, 2005. View at Publisher · View at Google Scholar · View at Scopus
  74. P. Sangsurasak and D. A. Mitchell, “Incorporation of death kinetics into a 2-dimensional dynamic heat transfer model for solid state fermentation,” Journal of Chemical Technology and Biotechnology, vol. 64, no. 3, pp. 253–260, 1995. View at Publisher · View at Google Scholar · View at Scopus
  75. M. T. Hardin, D. A. Mitchell, and T. Howes, “Approach to designing rotating drum bioreactors for solid-state fermentation on the basis of dimensionless design factors,” Biotechnology and Bioengineering, vol. 67, no. 3, pp. 274–282, 2000. View at Google Scholar · View at Scopus
  76. M. A. I. Schutyser, P. Pagter, F. J. Weber, W. J. Briels, R. M. Boom, and A. Rinzema, “Substrate aggregation due to aerial hyphae during discontinuously mixed solid-state fermentation with Aspergillus oryzae: experiments and modeling,” Biotechnology and Bioengineering, vol. 83, no. 5, pp. 503–513, 2003. View at Publisher · View at Google Scholar · View at Scopus
  77. M. M. Santos, A. S. Rosa, S. Dal'Boit, D. A. Mitchell, and N. Krieger, “Thermal denaturation: is solid-state fermentation really a good technology for the production of enzymes?” Bioresource Technology, vol. 93, no. 3, pp. 261–268, 2004. View at Publisher · View at Google Scholar · View at Scopus
  78. A. Aloulou, J. A. Rodriguez, D. Puccinelli et al., “Purification and biochemical characterization of the LIP2 lipase from Yarrowia lipolytica,” Biochimica et Biophysica Acta, vol. 1771, no. 2, pp. 228–237, 2007. View at Publisher · View at Google Scholar · View at Scopus
  79. M. L. Rua, C. Schmidt-Dannert, S. Wahl, A. Sprauer, and R. D. Schmid, “Thermoalkalophilic lipase of Bacillus thermocatenulatus: large-scale production, purification and properties: aggregation behaviour and its effect on activity,” Journal of Biotechnology, vol. 56, no. 2, pp. 89–102, 1997. View at Publisher · View at Google Scholar · View at Scopus
  80. M. Teissere, M. Borel, B. Caillol, J. Nari, A. M. Gardies, and G. Noat, “Purification and characterization of a fatty acyl-ester hydrolase from post-germinated sunflower seeds,” Biochimica et Biophysica Acta, vol. 1255, no. 2, pp. 105–112, 1995. View at Publisher · View at Google Scholar · View at Scopus
  81. H. Sztajer, H. Lünsdorf, H. Erdmann, U. Menge, and R. Schmid, “Purification and properties of lipase from Penicillium simplicissimum,” Biochimica et Biophysica Acta, vol. 1124, no. 3, pp. 253–261, 1992. View at Publisher · View at Google Scholar
  82. K. Isobe, K. Nokihara, S. Yamaguchi, T. Mase, and R. D. Schmid, “Crystallization and characterization of monoacylglycerol and diacylglycerol lipase from Penicillium camembertii,” European Journal of Biochemistry, vol. 203, no. 1-2, pp. 233–237, 1992. View at Google Scholar · View at Scopus
  83. A. G. Cunha, G. F. Lorente, M. L. E. Gutarra et al., “Separation and immobilization of lipase from Penicillium simplicissimum by selective adsorption on hydrophobic supports,” Applied Biochemistry and Biotechnology, vol. 156, no. 1–3, pp. 133–145, 2009. View at Publisher · View at Google Scholar · View at Scopus
  84. R. K. Saxena, A. Sheoran, B. Giri, and W. S. Davidson, “Purification strategies for microbial lipases,” Journal of Microbiological Methods, vol. 52, no. 1, pp. 1–18, 2003. View at Publisher · View at Google Scholar · View at Scopus
  85. P. D. María, J. M. Sánchez-Montero, J. V. Sinisterra, and A. R. Alcântara, “Understanding Candida rugosa lipases: an overview,” Biotechnology Advances, vol. 24, no. 2, pp. 180–196, 2006. View at Publisher · View at Google Scholar · View at Scopus
  86. P. F. F. Amaral, Produção de lipase de Yarrowia lipolytica em biorreator multifásico, Ph.D. thesis, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil, 2007.
  87. F. V. P. Meirelles, Produção de lipase de Yarrowia lipolytica (IMUFRJ50682), Ph.D. thesis, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil, 1997.
  88. M. F. C. P. J. S. Gama, Produção e caracterização de lipases de Penicillium restrictum, Dissertation. thesis, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil, 2000.
  89. T. S. M. Martins, Produção e purificação de lipases de Yarrowia lipolytica (IMUFRJ50682), Dissertation. thesis, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil, 2001.
  90. R. V. Almeida, Clonagem, expressão, caracterização e modelagem estrutural de uma esterase termoestável de Pyrococcus furiosus, Ph.D. thesis, University of Rio de Janeiro, Rio de Janeiro, Brazil, 2005.
  91. A. Pandey, Handbook of Plant-Based Biofuels, CRC Press, Boca Raton, Fla, USA, 2009.
  92. P. A. Z. Suarez, A. L. F. Santos, J. P. Rodrigues, and M. B. Alves, “Biocombustíveis a partir de óleos e gorduras: desafios tecnológicos para viabilizá-los,” Química Nova, vol. 32, no. 3, pp. 768–775, 2009. View at Google Scholar
  93. C. M. Drapcho, N. P. Nhuan, and T. H. Walker, Biofuels Engineering Process Technology, McGraw-Hill, New York, NY, USA, 2008.
  94. R. C. Rodrigues, Síntese de biodiesel através de transesterificação enzimática de óleos vegetais catalisada por lipase imobilizada por ligação covalente multipontual, Ph.D. thesis, Federal University of Rio Grande do Sul, Porto Alegre, Brazil, 2009.
  95. M. Sadeghi, “Purification of soybean oil with phospholipase Al,” Theoretical and Experimental Chemistry, vol. 46, no. 2, pp. 132–137, 2010. View at Google Scholar · View at Scopus
  96. B. Yang, R. Zhou, J. G. Yang, Y. H. Wang, and W. F. Wang, “Insight into the enzymatic degumming process of soybean oil,” Journal of the American Oil Chemist's Society, vol. 85, no. 5, pp. 421–425, 2008. View at Publisher · View at Google Scholar · View at Scopus
  97. K. Clausen, “Enzymatic oil-degumming by a novel microbial phospholipase,” European Journal of Lipid Science and Technology, vol. 103, no. 6, pp. 333–340, 2001. View at Google Scholar · View at Scopus
  98. L. De Maria, J. Vind, K. M. Oxenboll, A. Svendsen, and S. Patkar, “Phospholipases and their industrial applications,” Applied Microbiology and Biotechnology, vol. 74, no. 2, pp. 290–300, 2007. View at Publisher · View at Google Scholar · View at Scopus
  99. S. K. Roy, B. V. S. K. Rao, and R. B. N. Prasad, “Enzymatic degumming of rice bran oil,” Journal of the American Oil Chemist's Society, vol. 79, no. 8, pp. 845–846, 2002. View at Google Scholar · View at Scopus
  100. T. Tan, J. Lu, K. Nie, L. Deng, and F. Wang, “Biodiesel production with immobilized lipase: a review,” Biotechnology Advances, vol. 28, no. 5, pp. 628–634, 2010. View at Publisher · View at Google Scholar · View at Scopus
  101. P. M. Nielsen, J. Brask, and L. Fjerbaek, “Enzymatic biodiesel production: technical and economical considerations,” European Journal of Lipid Science and Technology, vol. 110, no. 8, pp. 692–700, 2008. View at Publisher · View at Google Scholar · View at Scopus
  102. A. Robles-Medina, P. A. Gonzalez-Moreno, L. Esteban-Cerdan, and E. Molina-Grima, “Biocatalysis: towards ever greener biodiesel production,” Biotechnology Advances, vol. 27, no. 4, pp. 398–408, 2009. View at Publisher · View at Google Scholar · View at Scopus
  103. W. Du, W. Li, T. Sun, X. Chen, and D. Liu, “Perspectives for biotechnological production of biodiesel and impacts,” Applied Microbiology and Biotechnology, vol. 79, no. 3, pp. 331–337, 2008. View at Publisher · View at Google Scholar · View at Scopus
  104. W. Soetaert and E. J. Vandamme, Biofuels, John Wiley & Sons, Hoboken, NJ, USA, 2009.
  105. S. V. Ranganathan, S. L. Narasimhan, and K. Muthukumar, “An overview of enzymatic production of biodiesel,” Bioresource Technology, vol. 99, no. 10, pp. 3975–3981, 2008. View at Publisher · View at Google Scholar · View at Scopus
  106. C. Z. Brusamarelo, E. Rosset, A. Césaro et al., “Kinetics of lipase-catalyzed synthesis of soybean fatty acid ethyl esters in pressurized propane,” Journal of Biotechnology, vol. 147, no. 2, pp. 108–115, 2010. View at Publisher · View at Google Scholar · View at Scopus
  107. A. L. F. Matassoli, I. N. S. Corrêa, M. F. Portilho, C. O. Veloso, and M. A. P. Langone, “Enzymatic synthesis of biodiesel via alcoholysis of palm oil,” Applied Biochemistry and Biotechnology, vol. 155, no. 1–3, pp. 347–355, 2009. View at Publisher · View at Google Scholar · View at Scopus
  108. Y.-D. Wang, X.-Y. Shen, Z.-L. Li et al., “Immobilized recombinant Rhizopus oryzae lipase for the production of biodiesel in solvent free system,” Journal of Molecular Catalysis B, vol. 67, no. 1-2, pp. 45–51, 2010. View at Publisher · View at Google Scholar
  109. K. Ban, M. Kaieda, T. Matsumoto, A. Kondo, and H. Fukuda, “Whole cell biocatalyst for biodiesel fuel production utilizing Rhizopus oryzae cells immobilized within biomass support particles,” Biochemical Engineering Journal, vol. 8, no. 1, pp. 39–43, 2001. View at Publisher · View at Google Scholar · View at Scopus
  110. T. F. C. Salum, P. Villeneuve, B. Barea et al., “Synthesis of biodiesel in column fixed-bed bioreactor using the fermented solid produced by Burkholderia cepacia LTEB11,” Process Biochemistry, vol. 45, no. 8, pp. 1348–1354, 2010. View at Publisher · View at Google Scholar · View at Scopus
  111. H. Fukuda, S. Hama, S. Tamalampudi, and H. Noda, “Whole-cell biocatalysts for biodiesel fuel production,” Trends in Biotechnology, vol. 26, no. 12, pp. 668–673, 2008. View at Publisher · View at Google Scholar · View at Scopus
  112. M. M. R. Talukder, J. C. Wu, and L. P. L. Chua, “Conversion of waste cooking oil to biodiesel via enzymatic hydrolysis followed by chemical esterification,” Energy and Fuels, vol. 24, no. 3, pp. 2016–2019, 2010. View at Publisher · View at Google Scholar · View at Scopus
  113. E. D. Cavalcanti-Oliveira, P. R. R. Silva, A. P. Ramos, D. A. G. Aranda, and D. M. G. Freire, “Study of soybean oil hydrolysis catalyzed by Thermomyces lanuginosus lipase and its application to biodiesel production via hydroesterification,” Enzyme Research, vol. 2011, Article ID 618692, 8 pages, 2011. View at Publisher · View at Google Scholar
  114. J. S. Sousa, E. D. Cavalcanti-Oliveira, D. A. G. Aranda, and D. M. G. Freire, “Application of lipase from the physic nut (Jatropha curcas L.) to a new hybrid (enzyme/chemical) hydroesterification process for biodiesel production,” Journal of Molecular Catalysis B, vol. 65, no. 1–4, pp. 133–137, 2010. View at Publisher · View at Google Scholar · View at Scopus
  115. W. Xie and N. Ma, “Enzymatic transesterification of soybean oil by using immobilized lipase on magnetic nano-particles,” Biomass and Bioenergy, vol. 34, no. 6, pp. 890–896, 2010. View at Publisher · View at Google Scholar · View at Scopus
  116. A. P. Vyas, J. L. Verma, and N. Subrahmanyam, “A review on FAME production processes,” Fuel, vol. 89, no. 1, pp. 1–9, 2010. View at Publisher · View at Google Scholar · View at Scopus
  117. G. Kuhn, M. Marangoni, D. M. G. Freire et al., “Esterification activities of non-commercial lipases after pre-treatment in pressurized propane,” Journal of Chemical Technology and Biotechnology, vol. 85, no. 6, pp. 839–844, 2010. View at Publisher · View at Google Scholar