Enzyme Mechanism and Slow-Onset Inhibition of Plasmodium falciparum Enoyl-Acyl Carrier Protein Reductase by an Inorganic Complex
Figure 4
Overall dissociation constant for PfENR: NADH (a) and PfENR : butyryl-CoA (b) binary complex formation monitoring changes in, respectively, nucleotide and intrinsic protein fluorescence. (c) Hill logarithmic plot of the data between 10% and 90% active site saturation with butyryl-CoA. Inset represents the fit of double-reciprocal plot of the fluorescence data to an exponential growth equation.
