Enzyme Mechanism and Slow-Onset Inhibition of Plasmodium falciparum Enoyl-Acyl Carrier Protein Reductase by an Inorganic Complex
Figure 5
Representative stopped-flow trace for NADH→NAD+ conversion. Fitting the exponential decay data to (5) yielded a value of 1100 (±29) s−1 for the apparent first-order rate constant of product formation. The top trace represents the chemical control (200 μM NADH), and the single exponential decay represents the reaction upon mixing 3.14 μM PfENR and 200 μM NADH (mixing chamber concentrations).
