The Sphingolipid Biosynthetic Pathway Is a Potential Target for Chemotherapy against Chagas Disease

Table 1

Genes required for sphingolipid biosynthesis and lipid remodeling steps in mammals, Saccharomyces cerevisiae, Trypanosoma brucei, Leishmania major, and Trypanosoma cruzi.

Step	Activity	Mammals	S. cerevisiae	T. brucei	L. major	T. cruzi
1	SPT	LCB1; LCB2	LCB1/LCB2; TSC3	TbSPT1; TbSPT2	LmSPT1; LmSPT2	TcSPT1; TcSPT2
2	KDSR	FVT-1	TSC10	Tb927.10.4040 ^(a)	LmjF.35.0330	Tc00.1047053510997.10; Tc00.1047053506959.64 ^(a)
3	CERS	CERS1-6	LAG1/LAC1; LIP1	Tb927.8.7730; Tb927.4.4740	LmjF.31.1780	TcCERS1
4	SLS	SMS1; SMS2	AUR1; KEI1	TbSLS1-4	LmIPCS	TcIPCS1; TcIPCS2
5	ID	PGAP1	BST1	GPIdeAc2	—^(b)	Tc00.1047053508153.1040 ^(a)
6	GPIPLA2	PGAP3	PER1	—	—	—
7	LGPIAT-I	PGAP2	GUP1	TbGUP1	LmjF.19.1000/LmjF.19.1320; LmjF.19.1340/LmjF.19.1345; LmjF.19.1347^(a)	TcGUP1 ^(c)
8	CR	—	CWH43	—	LmjF27.1770 ^(a)	Tc00.1047053504153.120 ^(a)

^(a)Corresponding homologues of each yeast gene were found by BLAST at NCBI and GeneDB (http://www.genedb.org/).
^(b)Not found.
^(c)TcGUP can be Tc00.1047053508943.4, Tc00.1047053511355.59, or Tc00.1047053503809.90.