Trypanosoma cruzi Coexpressing Ornithine Decarboxylase and Green Fluorescence Proteins as a Tool to Study the Role of Polyamines in Chagas Disease Pathology
Figure 1
Growth kinetics of Y-GFP and Y-GFP-ODC clone cultured at different conditions. Cultures carried out under the indicated conditions were diluted to cells/mL when the stationary phase was reached. (a) Y-GFP cultured in rich media BHT (- -⬤- -), Diamond (—■—), and in Diamond supplemented with 2 mg/mL glucose (- -△- -). (b) Y-GFP cultured in the semisynthetic medium SDM79 without (— ◆ —) or with (- -◊- -) 1 mM putrescine. (c) Y-GFP-ODC clone cultured in SDM79 (—⬤—) or in SDM79 supplemented with 5 mM α-difluoromethylornithine (DFMO) (- -⬤- -). All cultures were continuously maintained in presence of 250 μg/mL G418.
