Trypanosoma cruzi Coexpressing Ornithine Decarboxylase and Green Fluorescence Proteins as a Tool to Study the Role of Polyamines in Chagas Disease Pathology
Figure 5
Metacyclogenesis of Y-GFP and Y-GFP-ODC strain and clone. After metacyclogenesis protocol (see details in Materials and Methods), the total mixed suspensions of E and MT from each condition (Control, TAU, or TAU + DFMO) were used to infect Vero cells for 24 h followed by detection of the host cell actin cytoskeleton as indicated under Materials and Methods. (a) Confocal images showing Vero cells infected for 24 h with T. cruzi Y-GFP and Y-GFP-ODC clone obtained from TAU medium. (b) Percentage of infected Vero cells with T. cruzi Y-GFP, T. cruzi Y-GFP-ODC strain, and T. cruzi Y-GFP-ODC clone at indicated conditions. Data represent the mean ± SEM of at least two independent experiments (number of counted cells ≈100). Significantly different from control: **, Bars: 10 μm.
