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Enzyme Research
Volume 2011, Article ID 679624, 8 pages
http://dx.doi.org/10.4061/2011/679624
Research Article

Amylolytic Microorganism from São Paulo Zoo Composting: Isolation, Identification, and Amylase Production

1Biological Science Department, Federal University of São Paulo, Rua Arthur Riedel, 275, 09972-270 Diadema, SP, Brazil
2Applied Microbiology Laboratory, Foundation of São Paulo Zoological Park, Rua Miguel Stéfano 4241, 04301-905 São Paulo, SP, Brazil
3Biophysics Department, Federal University of São Paulo, Rua 3 de Maio, 100, 04044-020 São Paulo, SP, Brazil

Received 10 January 2011; Revised 24 April 2011; Accepted 9 June 2011

Academic Editor: D. M. G. Freire

Copyright © 2011 Renata C. Pascon et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Composting is a way of transforming the organic waste into fertilizer, minimizing the use of inorganic compounds that may contaminate the environment. This transformation is the result of the microorganism action, converting complex carbon sources into energy. Enzymes that are exported by the microorganisms to the surrounding environment mediate this process. The aiming of the present work is to prospect the compost produced by the organic composting unit (OCU) of the Fundação Parque Zoológico de São Paulo (FPZSP) to find novel starch hydrolyzing organisms (SHO) that secrete large amounts of amylases under harsh conditions, such as high temperature. We found five bacterial isolates that have amylolytic activity induced by soluble starch and 39°C temperature of growth. These bacterial strains were identified by MALDI-TOF (Matrix-assisted laser desorption/ionization-Time of Flight) analysis, a rapid and efficient methodology for microbe identification in large scale. Our results present amylolytic strains that belong to diverse taxonomic groups (Solibacillus silvestris, Arthrobacter arilaitensis, Isoptericola variabilis, and Acinetobacter calcoaceticus); some of them have never been associated with this kind of hydrolytic activity before. The information regarding enzyme induction will be important to optimize the production by the bacterial isolates, which may be a great value for biotechnological applications.