Amylolytic Microorganism from São Paulo Zoo Composting: Isolation, Identification, and Amylase Production

Table 3

Maximum specific activity (U/mg of protein) and the total units produced at the time point (hours) where the highest specific activity was found for each of the 5 isolates that showed enzymatic induction. 30°C and 39°C are the temperatures of growth where supernatant samples were collected to conduct the enzymatic assays. LB indicates the complex medium used, and starch refers to the induction medium containing starch as the major carbon source, in addition to a trace of peptone. All data points are average of 3 replicates of growth curves and 3 replicates of enzyme assay per time point (equals to 9 replicates).

Strain	30°C						39°C
	LB			Starch			LB			Starch
	U/mg	Total units	Time point	U/mg	Total units	Time point	U/mg	Total units	Time point	U/mg	Total units	Time point
URX291	0.079 ± 0.007	4	6	0.022 ± 0.001	4.3	16	0.043 ± 0.002	5.9	18	0.114 ± 0.016	8.2	6
URX303	0.098 ± 0.002	6.8	18	0.015 ± 0.002	2.7	16	0.036 ± 0.011	4.5	16	0.278 ± 0.024	25.2	26
URX350	0.097 ± 0.007	6.5	16	0.022 ± 0.004	2.2	16	0.037 ± 0.001	4.7	16	0.134 ± 0.014	19.4	18
UED641	0.094 ± 0.005	6.1	16	0.029 ± 0.002	2.9	4	0.035 ± 0.001	4.4	16	0.120 ± 0.006	8.7	2
UED644	0.160 ± 0.027	9.2	24	0.037 ± 0.003	3.2	2	0.035 ± 0.001	4.3	16	0.230 ± 0.024	19.4	2