Table of Contents Author Guidelines
Enzyme Research
Volume 2011, Article ID 692618, 11 pages
http://dx.doi.org/10.4061/2011/692618
Research Article

Enzymatic Synthesis of the Flavone Glucosides, Prunin and Isoquercetin, and the Aglycones, Naringenin and Quercetin, with Selective -L-Rhamnosidase and -D-Glucosidase Activities of Naringinase

1Research Institute for Medicines and Pharmaceutical Sciences (i-Med-UL), Faculty of Pharmacy, University of Lisbon, Avenue Prof. Gama Pinto, 1649-003 Lisbon, Portugal
2Departmento de Química, Instituto de Tecnologia Química e Biológica, Apartado 127, Av. República, 2784-505 Oeiras, Portugal

Received 21 April 2011; Accepted 8 July 2011

Academic Editor: J. Guisan

Copyright © 2011 Hélder Vila-Real et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The production of flavonoid glycosides by removing rhamnose from rutinosides can be accomplished through enzymatic catalysis. Naringinase is an enzyme complex, expressing both -L-rhamnosidase and -D-glucosidase activities, with application in glycosides hydrolysis. To produce monoglycosylated flavonoids with naringinase, the expression of -D-glucosidase activity is not desirable leading to the need of expensive methods for -L-rhamnosidase purification. Therefore, the main purpose of this study was the inactivation of -D-glucosidase activity expressed by naringinase keeping -L-rhamnosidase with a high retention activity. Response surface methodology (RSM) was used to evaluate the effects of temperature and pH on -D-glucosidase inactivation. A selective inactivation of -D-glucosidase activity of naringinase was achieved at C and pH 3.9, keeping a very high residual activity of -L-rhamnosidase (78%). This was a crucial achievement towards an easy and cheap production method of very expensive flavonoids, like prunin and isoquercetin starting from naringin and rutin, respectively.