Research Article

Enzyme-Catalyzed Oxidation of 17β-Estradiol Using Immobilized Laccase from Trametes versicolor

Figure 5

Effect of changing temperature on E2 conversion accomplished by immobilized enzyme for temperature changes (a) from ambient (21°C) to cold (3°C) and back to ambient, and (b) from ambient to warm (33°C) and back to ambient. Reaction conditions: 8 g, 18.0 U/g nominal activity, pH 5.
(a) Cold step change
(b) Warm step change