Immobilization of Erwinia sp. D12 Cells in Alginate-Gelatin Matrix and Conversion of Sucrose into Isomaltulose Using Response Surface Methodology

Table 2

Main effects and interactions of immobilization parameters of Erwinia sp. D12 cells in calcium alginate and their influence on converting sucrose into reducing sugars in a batch process.

	Effect	Standard error	𝑡 (3)	P
(1) Sodium alginate (L)*	31.3234	6.5836	4.7578	0.0176
Sodium alginate (Q)	−3.6308	6.8480	−0.5302	0.6327
(2) Cell mass (L)*	179.5066	6.5836	27.2655	0.0001
Cell mass (Q)*	99.9771	6.8480	14.5996	0.0007
(3) CaCl2 (L)*	136.1412	6.5836	20.6787	0.0002
CaCl2 (Q)*	23.4725	6.8480	3.4277	0.0416
(1)L × (2)L	−19.0278	8.5982	−2.2130	0.1138
(1)L × (3)L*	34.6544	8.5982	4.0304	0.0275
(2)L × (3)L*	81.8471	8.5982	9.5191	0.0025

*Statistically significant parameters at a 95% confidence level.
L: linear parameter; Q: quadratic parameter.