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Enzyme Research
Volume 2011, Article ID 796394, 6 pages
http://dx.doi.org/10.4061/2011/796394
Research Article

Improvement of Phytase Activity by a New Saccharomyces cerevisiae Strain Using Statistical Optimization

Food Science Department, Faculty of Food Engineering, Campinas State University (UNICAMP), Monteiro Lobato Street 70, 13083 970 Campinas, SP, Brazil

Received 21 March 2011; Revised 18 June 2011; Accepted 21 June 2011

Academic Editor: Alane Beatriz Vermelho

Copyright © 2011 Edi Franciele Ries and Gabriela Alves Macedo. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Using statistical optimization, we enhanced the activity of phytase by a new Saccharomyces cerevisiae strain cultured in mineral medium. Concentrations of carbon source and inducer of phytase production were optimized using a 22 full factorial CCD and response surface methodology (RSM). Urea was fixed as nitrogen source in culture medium (0.15%, w/v). The culture medium consisting of 2.5% sucrose and 0.5% sodium phytate optimally supported the maximum phytase activity. In addition, we found that culture of the yeast at 35°C with shaking at 150 rpm supports maximum phytase production. The validity of this model was verified by culturing the organisms in flasks on a shaker. Using the optimized media and growth conditions, we obtained a 10-fold improvement in the production of phytase by S. cerevisiae.