Cross-Linked Enzyme Aggregates of Naringinase: Novel Biocatalysts for Naringin Hydrolysis

Table 2

Effects and respective significance levels (𝑝) of [Naringin] and temperature (𝑇) on reducing sugars formation and naringinase residual activity (𝐴_𝑟).

Factor	[Reducing sugars] (mg/mL)		Activity (mg/mL·min)
		(𝑝)		(𝑝)
[Naringin] (linear)	0.3471	0.0094	11.578	0.0094
[Naringin] (quadratic)	−0.2340	0.0666	−7.804	0.0665
Temperature (linear)	0.2381	0.0475	7.944	0.0477
Temperature (quadratic)	−0.4551	0.0107	−15.175	0.0107
[Naringin] × temperature	0.1555	0.2491	5.175	0.2497

* Naringin hydrolysis in acetate buffer (20 mM) pH 4.0 with NGase-CLEAs (NGase 2 mg/mL).