Enzyme Research

Enzyme Research / 2011 / Article / Tab 2

Research Article

Cross-Linked Enzyme Aggregates of Naringinase: Novel Biocatalysts for Naringin Hydrolysis

Table 2

Effects and respective significance levels ( ) of [Naringin] and temperature ( ) on reducing sugars formation and naringinase residual activity ( ).

Factor[Reducing sugars] (mg/mL)Activity (mg/mL·min)
( )( )

(linear)0.34710.009411.5780.0094
(quadratic)−0.23400.0666−7.8040.0665
Temperature (linear)0.23810.04757.9440.0477
Temperature (quadratic)−0.45510.0107−15.1750.0107
× temperature0.15550.24915.1750.2497

* Naringin hydrolysis in acetate buffer (20 mM) pH 4.0 with NGase-CLEAs (NGase 2 mg/mL).