Enolase: A Key Player in the Metabolism and a Probable Virulence Factor of Trypanosomatid Parasites—Perspectives for Its Use as a Therapeutic Target

Table 1

Kinetic characteristics of the natural and recombinant enolases of T. brucei, T. cruzi, and L. mexicana.

Source of enzyme	𝐾m		𝑉max
	(𝜇M)		(μmol·min^−1·mg^−1)
	2PGA	PEP	2PGA	PEP
T. brucei (natural)	54	244	ND	ND
T. brucei (recombinant)	49	289	63	6.3
T. cruzi (natural)	55	147	97	42
T. cruzi (recombinant)	50	410	0.87	12.35
L. mexicana (natural)	80	216	16.0	3.38
L. mexicana (recombinant)	51	200	80.0	3.64
Rabbit muscle	16	238	31	6.4
Yeast	57	264	65	7.8

Data of T. brucei, rabbit muscle, and yeast enolases were taken from Hannaert et al. [39], those of L. mexicana enolases from Quiñones et al. [40], and the T. cruzi data have not yet been reported before (L. González-González, W. Quiñones, and J. L. Concepción, unpublished results). Abbreviations: 2PGA: D-2-phosphoglycerate; PEP: phosphoenolpyruvate; ND: not determined.