Research Article

Kinetic Characterisation of Phosphofructokinase Purified from Setaria cervi: A Bovine Filarial Parasite

Figure 1

Effect of F-6-P concentration on the rate of S. cervi PFK catalyzed reaction. ATP concentration was 50 (○) and 100 (•) μM and Mg2+ concentration was 3.3 mM. Enzyme concentration was 6.6 μg/mL. Other conditions were same as in standard enzyme assay. (b) Double reciprocal plot of the data of (a). ATP concentrations were 50 (○) and 100 (•) μM.
939472.fig.001a
(a)
939472.fig.001b
(b)