Kinetic Characterisation of Phosphofructokinase Purified from Setaria cervi: A Bovine Filarial Parasite
Figure 3
Effect of F-6-P concentration on the rate of S. cervi PFK catalyzed reaction at different fixed and high concentration of ATP. ATP concentration was 0.1 (Δ), 0.33 (○), and 1.0 (•) mM and Mg2+ concentration was 3.3 mM. Enzyme concentration was 3.3 μg/mL. Other conditions were same as in standard enzyme assay. Inset. Hill plot of the data of (a) at 0.2–6.0 mM F-6-P concentrations. ATP concentrations were 0.1 (Δ), 0.33 (○), and 1.0 (•).
