Kinetic Characterisation of Phosphofructokinase Purified from Setaria cervi: A Bovine Filarial Parasite

Figure 4

Effect of ATP concentration on the rate of PFK catalyzed reaction at fixed concentration of F-6-P and Mg²⁺ (3.3 mM each). Inset shows the plot obtained on assaying the enzyme at variable inhibitory (>0.1 mM) concentration of ATP at two fixed concentrations of F-6-P such as 1.6 (•) and 3.3 (○) mM. Mg²⁺ concentration was constant (3.3 mM). Enzyme concentration was 10 μg/mL. Other conditions were same as in standard enzyme assay.