Kinetic Characterisation of Phosphofructokinase Purified from Setaria cervi: A Bovine Filarial Parasite
Figure 7
(a) Effect of FDP on the rate of PFK catalyzed reaction. FDP concentrations were nil (○), 1.0 (Δ), and 2.0 (▲) μM. Mg2+ concentration was constant (3.3 mM). The concentration of ATP was 0.1 mM. Enzyme concentration was 10 μg/mL. Other conditions were same as in standard enzyme assay. (b) Hill plot of the data of the effect of FDP on the rate of PFK catalyzed reaction. The concentrations of F-6-P, ATP, and Mg2+ were 3.3, 0.1, and 3.3 mM, respectively. Enzyme concentration was 6.6 μg/mL. and are the rates of reaction in the presence and absence of FDP. Other conditions were same as in standard enzyme assay.
